Zoology · Biotechnology: Principles and Processes
NEET (UG) 2025 — Question 163
Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
- Option A:Correct
C and D only
- Option B:
A and B only
- Option C:
D and E only
- Option D:
B and C only
Answer: A
Step-by-step solution
Gene cloning requires restriction enzymes, DNA ligase, and DNA polymerase for cutting, joining, and replication. DNA mutase is not a real enzyme and is not involved in standard gene cloning. DNA recombinase is not a standard enzyme used in gene cloning; recombination in cloning is achieved by restriction enzymes and ligase. Thus, the enzymes NOT essential for gene cloning are DNA mutase (C) and DNA recombinase (D). Therefore, the correct answer is option A: C and D only.
Answer key and solution verified before publishing.
Practise Biotechnology: Principles and Processes
Start with this question, then two more from the same chapter — with a tutor that explains every step. Free.
- Exam
- NEET (UG) 2025
- Paper
- 2025 paper
- Subject
- Zoology
- Chapter
- Biotechnology: Principles and Processes
- Topic
- Restriction Enzymes
More Biotechnology: Principles and Processes questions from this paper
- Polymerase chain reaction (PCR) amplifies DNA following the equation:
- In the plasmid represented above, a foreign piece of DNA is inserted at the EcoRI site. Which of the following strategies will be chosen to…
- Identify the part of a bioreactor which is used as a foam breaker from the given figure.
- Given below are two statements : one is labelled as Statement I and the other is labelled as Statement II: Statement I: The DNA fragments…
- Given below are two statements : one is labelled as Statement I and the other is labelled as Statement II: The blue and white selectable…