Zoology · Biotechnology: Principles and Processes

NEET (UG) 2024 — Question 173

The following diagram showing restriction sites in $E$ . coli cloning vector $p B R 322$ . Find the role of ' $X$ and ' $Y$ genes:

Option A:
The gene ' $X$ ' is responsible for resistance to antibiotics and ' $Y$ for protein involved in the replication of Plasmid.
Option B:
The gene ' $X$ ' is responsible for controlling the copy number of the linked DNA and ' $Y$ for protein involved in the replication of Plasmid.
Correct
Option C:
The gene ' $X$ ' is for protein involved in replication of Plasmid and ' $Y$ for resistance to antibiotics.
Option D:
Gene ' $X$ ' is responsible for recognitions sites and ' $Y$ ' is responsible for antibiotic resistance.

Answer: B

Correct answer is option (2), because

' $X$ ' in the given diagram is ori while ' $Y$ is rop.

' $X$ which is ori is responsible for controlling the copy number of the linked DNA and ' $\gamma$ which

is rop codes for protein involved in the replication of plasmid.

Options (1), (3) and (4) are incorrect as ' $X$ and ' $Y$ are not related to these functions.

Answer key and solution verified before publishing.

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